http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105907758-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-10043 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-80 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-861 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 |
| filingDate | 2016-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105907758-B |
| titleOfInvention | CRISPR-Cas9 guide sequence and primer thereof, transgenic expression vector and construction method thereof |
| abstract | The invention belongs to the technical field of biological medicines, and particularly relates to a CRISPR-Cas9 guide sequence primer, a transgenic expression vector and a construction method thereof. The CRISPR-Cas9 guide sequence sgRNA comprises Target site-1 located on E1 exon for a Target point of the SIDT1 gene and Target site-2 located on E2 exon for a Target point of the SIDT1 gene; wherein, the nucleotide sequence of Target site-1 is shown as SEQ ID NO.8, and the nucleotide sequence of Target site-2 is shown as SEQ ID NO. 9. The CRISPR-Cas9 transgenic expression vector comprises a BsmB I enzyme cutting site and a Bbs I enzyme cutting site of a recombinant shuttle Cas9 tool plasmid which are connected with Cas9 guide sequences, Target site-1 and Target site-2. The recombinant shuttle cas9 tool plasmid can be rapidly assembled with guide sequences aiming at two target sites of a genome to be further packaged into a recombinant adenovirus vector aiming at the two target sites, and can independently finish the editing of a large-fragment genome without depending on a synergistic effect; and the construction process is simple and quick, and the efficiency of playing the editing role of large fragments of the genome is high. |
| priorityDate | 2016-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 185.