http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105866352-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2033-0091 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2001-2866 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-286 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-00 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-00 |
filingDate | 2016-06-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2018-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2018-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-105866352-B |
titleOfInvention | Genetically modified plants pollen is evaluated to the method for honeybee toxicological effect and pollen extracting liquid and preparation method thereof |
abstract | The invention discloses a kind of evaluation genetically modified plants pollen to the method for honeybee toxicological effect and pollen extracting liquid and preparation method thereof, evaluation method includes:The pollen extracting liquid of prepare transgenosis and same kind non-transgenic plant respectively;The preparation of pollen extracting liquid includes:Herborization pollen, crushes, and adds Extraction buffer and carries out concussion extraction, is centrifuged after the completion of extraction, supernatant is pollen extracting liquid;Extraction buffer is by certain density KH 2 PO 4 、Na 2 HPO 4 , NaCl, KCl and polysorbas20 composition;Sucrose is added into pollen extracting liquid, feeding liquid is made;Using feeding liquid feeding honeybee, compare bee death rate, judge toxicity of the genetically modified plants pollen to honeybee.The present invention can simulate the truth of honeybee feeding transgenic pollen in the case of nature. |
priorityDate | 2016-06-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 34.