http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105866053-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-33 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-33 |
| filingDate | 2016-04-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105866053-B |
| titleOfInvention | A kind of method of double enzyme detection γ-Gamma-propalanine contents |
| abstract | The present invention provides a kind of methods that double enzymes detect γ-Gamma-propalanine content.This approach includes the following steps：Grinding waits for test sample in liquid nitrogen, and alcohol is added；Vacuum drying is added lanthanum and collects supernatant after mixing centrifugation；Lye is added after mixing centrifugation to supernatant again and obtains γ-aminobutyric acid extracting solution；NADP is added into gained extracting solution again + , Tris buffer solutions, the double enzyme solutions of γ-aminobutyric acid transaminase succinic semialdehyde dehydrogenase, obtain mixed liquor；Using ultraviolet-uisible spectrophotometer, initial value of the above-mentioned mixed liquor at 340nm wavelength is measured；Then alpha Ketoglutarate is added into mixed liquor to be reacted, measures end value of the reaction solution at 340nm wavelength, draws standard curve, acquire a regression curve；According to initial value, end value and a regression curve, the content of γ-aminobutyric acid is calculated.The present invention can effectively reduce interference, the simple and quick content for accurately detecting γ-aminobutyric acid in plant and animal material. |
| priorityDate | 2016-04-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 59.