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filingDate 2016-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2019-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2019-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-105861444-B
titleOfInvention The cell line and its application of one plant of anti-B subgroup avian leucosis virus
abstract The present invention provides the cell line and its application of one plant of anti-B subgroup avian leucosis virus (ALV-B).ALV-B env gene is inserted into expression vector, the transfer vector of building is expressed in chicken embryo fibroblasts system DF-1, the chicken embryo fibroblasts system DF-1/B for having Zeocin resistance, stablizing expression ALV-B env gene is obtained by Zeocin drug screening, which is CCTCC No.C201609.Detection and viral infection resisting analysis are carried out to DF-1/B cell line, it is found that the ALV-B env albumen expressed in DF-1 cell line has superinfection resistance to ALV-B virus, can resist titre is 10 4 TCID 50 Virus infection duplication amount, DF-1/B cell line of the invention can be used for the antidiastole of different subgroup avian leucosis virus infection, and diagnostic value is significant, has a extensive future.
priorityDate 2016-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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