http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105842447-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5764 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-582 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-576 |
filingDate | 2016-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2017-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2017-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-105842447-B |
titleOfInvention | A kind of detection method for hepatitis B surface antigen |
abstract | The invention provides a kind of detection method for hepatitis B surface antigen, this method is first combined hepatitis B surface antigen with coated polyclonal antibody, then biotinylated monoclonal antibody is connected, reconnect the catalase C100 of marked by streptavidin, decomposing hydrogen dioxide solution is catalyzed by catalase, the fluorescent quenching of the cadmium telluride quantum dot to mercaptopropionic acid modification is reduced, according to the height of fluorescence intensity come the concentration of hepatitis B surface antigen in judgement sample.This method is based on double antibodies sandwich Enzyme-multiplied immune technique, and employ biotin avidin system be used for react amplification.What is more important, due to present invention employs new antibody labeling enzyme (catalase C100) and more sensitive fluorogenic substrate (cadmium telluride quantum dot), and it have matched effective reaction condition, so that detection sensitivity is significantly improved, cost, lifting detection efficiency are reduced simultaneously, therefore there is good promotion prospect. |
priorityDate | 2016-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 187.