http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105838668-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-392 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-30 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0609 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-075 |
| filingDate | 2016-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-05-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-05-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105838668-B |
| titleOfInvention | In-vitro maturation culture solution for small follicle oocytes and application thereof |
| abstract | The invention relates to cell culture, and particularly discloses an in-vitro maturation culture solution for a small oocyte, which contains 100nM E2 and 100ng/mL CNP/BNP. The invention also provides application of the in vitro maturation culture solution in promoting in vitro maturation of the small follicular oocyte or inhibiting meiosis of the small follicular oocyte, and a culture method for promoting in vitro maturation of the oocyte, which specifically comprises the following steps: oocytes from animals were collected and classified according to size of follicular diameter: placing the small oocyte in the in vitro maturation culture solution for pre-maturation culture, wherein the culture time is 20h, and then culturing in the maturation culture solution for 46 +/-2 h; the large follicle oocytes were cultured directly in maturation medium for 46 + -2 h. The invention adopts two-section in vitro maturation culture method, applies CNP/BNP and E2 to perform pre-maturation treatment on the small follicle oocyte, promotes synchronization of nuclear maturation and cytoplasm maturation, improves in vitro developmental capacity, and can become a novel meiosis inhibition drug to be popularized and applied in animal husbandry production. |
| priorityDate | 2016-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 64.