| abstract |
Provided are host cells engineered to produce benzylisoquinoline alkaloid (BIA) precursors such as higenamine (NC) and pernordane (NL). The host cell may have one or more engineered modifications selected from the group consisting of feedback inhibition mitigating mutations in enzyme genes; transcriptional regulatory modifications in biosynthetic enzyme genes; inactivating mutations in enzymes; and heterologous coding sequences . Also provided are methods of producing a target BIA or a precursor thereof using the host cells and compositions, eg, kits, systems, etc., suitable for use in the methods of the present invention. |