http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105821120-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6848 |
| filingDate | 2016-02-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-04-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-04-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105821120-B |
| titleOfInvention | A kind of T790M mutation detection methods based on the compound COLD-PCR of nido |
| abstract | The invention discloses a kind of T790M mutation detection methods based on the compound COLD-PCR joint sequencing of nido.This method carries out the compound COLD-PCR joint sequencing of nido using sample to be tested nucleic acid as template, using primer sets shown in NO.1 ~ 4 SEQ ID and NO.5 ~ 8 SEQ ID.The compound COLD-PCR of nido includes outer reaction and interior reaction, and outer reaction is the composite reaction of COLD-PCR and routine PCR reaction, and interior reaction is routine PCR reaction.This method has good abrupt climatic change sensitivity, Monitoring lower-cut and repeatability, and indifference amplification wild type and mutant sample and same there can be mutation concentration effect, it can also be by adjusting the recurring number ratio of outer reaction, adjustment mutation concentration effect and detection sensitivity as needed, have extraordinary application prospect. |
| priorityDate | 2016-02-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.