http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105802943-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2457 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01041 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-84 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-44 |
| filingDate | 2016-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105802943-B |
| titleOfInvention | A kind of pichia pastoris yeast mutant strain of the Pullulanase chimera and high yield of the performance improvement chimera |
| abstract | The pichia pastoris yeast mutant strain of the Pullulanase chimera and high yield of a kind of the performance improvement chimera, belongs to microorganism, enzyme engineering technology and starch manufacture field.The present invention is to deriving from Bacillus deramificans With Bacillus naganoensis Pullulanase encoding gene carry out codon optimization, carry out molecule reorganization using DNA Shuffling, obtain a kind of dna chimeric gene, it is living that chimera enzyme is able to maintain high enzyme in acid condition, while specific enzyme activity is higher than and derives from Bacillus deramificans Pullulanase.The gene of encoding chimera body WXP03 Pullulanase enzyme activity is given expression in P. pastoris cell, 55 DEG C of recombinase optimum temperature, about 18 hours 4.5 enzyme activity half-life period of optimal pH, at 55 DEG C, pH4.0 enzyme activity half-life period is about 12 hours.Further mutagenesis screening obtains the pichia pastoris yeast mutant strain that one plant of shake-flask fermentation enzyme activity level significantly improves.Fermentation enzyme activity of the mutant strain on 5 L fermentors is up to 1800 U/mL. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113667662-A |
| priorityDate | 2016-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 514.