http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105802935-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P41-001 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P41-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-62 |
filingDate | 2016-05-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2019-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2019-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-105802935-B |
titleOfInvention | A kind of esterase PHE14 and its encoding gene and application |
abstract | The invention discloses a kind of esterase PHE14 and its encoding gene and applications.The present invention develops from marine pseudomonas (Pseudomonadaceae oryzihabitans) HUP022 and obtains new esterase gene PHE14, overall length 645bp, and the esterase PHE14 of coding contains 214 amino acid.Pass through clone esterase gene PHE14 and connects expression vector pET-28a (+) and convert e. coli bl21 (DE3) afterwards, after cultivating simultaneously inducing expression, the esterase PHE14 that is recombinantly expressed.Esterase PHE14 can be used for preparing chiral methyl lactate, and using the esterase PHE14 of recombinant expression as catalyst, (S)-methyl lactate that optical purity is greater than 99% has been prepared.Esterase PHE14 has very big application value in the fields such as biochemical industry and biological medicine. |
priorityDate | 2016-05-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 71.