| abstract |
The present invention provides a HPLC method for determining the content of chlorogenic acid and ammonium glycyrrhizinate in Yinma Jiedu granule, aiming at providing a method with more accurate measurement and higher detection sensitivity for determining chlorogenic acid and licorice in Yinma Jiedu granule The method of ammonium acid content; this method is to weigh 1g of the sample and place it in a 10mL extraction pool, use methanol to extract the sample statically at a temperature of 60°C for 3min, repeat the extraction twice, and after the extraction is completed, dilute the extract to 20mL with methanol , draw 1mL into a centrifuge tube, vortex centrifuge for 2min, take the supernatant and inject it into a high performance liquid chromatograph; it belongs to the technical field of chemical detection. |