| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cb351a5a4e03ee3773bd0df5dbae9ac3 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-26
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-30
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-301 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-30
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-26 |
| filingDate |
2016-04-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2a66c5438a4c193437afe7e15e9d471e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b217fd12f1629bf56643cc76e4f59b9a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f3a21c06c41b28d580b6e00f65a4d6e6 |
| publicationDate |
2016-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-105784799-A |
| titleOfInvention |
Electrochemical detection method for detecting kanamycin residues based on nucleic acid aptamer and nano analogue enzyme |
| abstract |
The invention provides an electrochemical detection method for detecting kanamycin residues based on a nucleic acid aptamer and a nano analogue enzyme and belongs to the technical field of analytical chemistry. Gold nano-particles are synthesized by reducing chloroauric acid through tyrosine, and hydrogen peroxide and reduced-state thionine are catalyzed to react to generate oxidized-state thionine; and the thionine can be detected through a difference pulse voltammetry. The gold nano-particles are modified through utilizing a kanamycin specific aptamer; and the aptamer is adsorbed on the surfaces of the gold nano-particles so that the peroxidase activity is inhibited. When a target object kanamycin exists, the aptamer can be competitively replaced from the surfaces of the gold nano-particles to form a compound, so that the peroxidase activity is recovered. The detection of the kanamycin can be realized through detecting a relation between a reduction peak current value of the oxidized-state thionine and an antibiotic concentration by utilizing the difference pulse voltammetry. The method provided by the invention has good repeatability, good stability and high sensitivity, and can be used for effectively detecting kanamycin residues in food samples. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110320356-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106841335-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108445031-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106841335-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107807117-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112082984-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107807117-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110687178-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110320356-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107703306-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113686929-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113686929-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107703306-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107356578-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110687178-A |
| priorityDate |
2016-04-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |