http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105779482-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-75 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-75 |
| filingDate | 2016-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-10-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-10-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105779482-B |
| titleOfInvention | A kind of bacillus gene based on dCas9 strikes low vector plasmid pBD1, building and application |
| abstract | The invention discloses a kind of bacillus genes based on dCas9 to strike low vector plasmid pBD1, building and application, the building of pBD1 plasmid, utilize the different kinds of molecules biological means such as PCR, digestion, enzyme company, sequencing, conversion, on the basis of bacillus expression vector pHY300p1k, construct the plasmid pHY300-dCas9-sgRNA (pBD1) of the Pgrac promoter containing IPTG induction type, dCas9 and sgRNA, because the promoter intensity of plasmid is big, has extensive host range, thus, theoretically the plasmid is suitable for extensive bacillus. |
| priorityDate | 2016-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 44.