http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105779389-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0634 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-078 |
| filingDate | 2016-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105779389-B |
| titleOfInvention | A kind of separation of megalobrama amblycephala peripheral white blood cells and primary culture method |
| abstract | The present invention relates to a kind of separation of megalobrama amblycephala peripheral white blood cells and primary culture methods, belong to the separation of animal peripheral blood leucocyte and culture field.It include animal hot housing, blood collection, leucocyte separation and culture and etc.: a certain amount of fish blood is conventionally extracted in gnotobasis, then using peripheral leukocytes in the method separation blood of Percoll gradient centrifugation, gained cell is subjected to washing purifying, and is placed in preference temperature and is cultivated；In the step, using the RPMI-1640 culture solution of improvement as culture medium, the Percoll separating liquid of various concentration is configured, cell is in 27 DEG C of cultivation temperature, 95% humidity, 5%CO 2 Carrying out originally culture 2h or more in environment can adherent growth.The well-grown peripheral white blood cells of megalobrama amblycephala can be obtained using the present invention. |
| priorityDate | 2016-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.