| abstract |
The invention discloses a kind of anti-BCMA Chimeric antigen receptor, encoding gene, recombinant expression carrier and its construction method and applications.The anti-BCMA Chimeric antigen receptor include include being sequentially connected in series including the CD8 leader Chimerical receptor signal peptide, BCMA single-chain antibody heavy chain VH, Optimal Linker C, BCMA single-chain antibody light chain VL, CD8 Hinge Chimerical receptor hinge, CD8 Transmembrane Chimerical receptor transmembrane region, CD137 Chimerical receptor costimulating factor and the TCR Chimerical receptor t cell activation domain that are sequentially connected in series.In addition, also disclosing encoding gene, recombinant expression carrier and its construction method and the application of the anti-BCMA Chimeric antigen receptor.The method can significantly improve the secretions of cell factor, the killing effect in vitro of CAR-T cell, and clinical therapeutic efficacy is prominent. |