| abstract |
The present invention relates to methods for generating cell and tissue compositions suitable for therapeutic use in mammals in need of therapeutic cell or tissue treatment. In particular, the present invention relates to a method of culturing cells for use in therapy, the method comprising (i) culturing a sample of cells in a first cell culture medium comprising a first serum, serum replacement and/or serum fraction, and (ii) culturing the cells in a second cell culture medium comprising a second serum, serum replacement and/or serum fraction prior to harvesting the cells for use in therapy. |