http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105754932-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-84 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-067 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 |
| filingDate | 2016-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105754932-B |
| titleOfInvention | Method for extracting and culturing primary hepatocytes of carp |
| abstract | The invention discloses a method for extracting and culturing primary hepatocytes of carps, which is suitable for the technical field of bioengineering, wherein carps are selected for dissection, livers are aseptically taken and cut into pieces, cell suspensions are obtained by cell screening and filtration after being digested by trypsin, then the cell suspensions are purified by Percolll, and culture solution containing carp serum is added to obtain new cell suspensions; counting by using a blood bead counting plate, culturing and proliferating by using a plate-laying method under the optimal culture condition, and finally performing microscopic examination and identification on the obtained primary hepatocytes by using various methods. The purification and culture method of the primary carp hepatocyte directly and aseptically takes the carp liver, purifies the hepatocyte through Percolll, really obtains the isolated carp primary hepatocyte with large quantity and high purity through identification, replaces the bovine serum in the traditional culture medium with the carp serum, improves the activity of the extracted isolated cell, and lays a foundation for further developing the toxicity test and other experiments of the primary carp hepatocyte. |
| priorityDate | 2016-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 49.