http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105738169-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-28 |
| filingDate | 2014-12-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105738169-B |
| titleOfInvention | A kind of protein N-terminal enrichment method and its application |
| abstract | The present invention relates to a kind of protein N end enrichment methods, including：The guanidinated label of selectivity, phosphate-labeled, phosphate radical affinitive material enrichment.Protein example uses alkaline phosphatase to remove phosphorylation posttranslational modification first, then the guanidinated label for carrying out selectivity to protein side chains amino under strongly alkaline conditions closes side-chain amino group, remaining albumen terminal amino group, which uses the amino active agent with phosphate radical to be marked, makes end take phosphoric acid root label, the albumen of label is digested, affine N end peptide fragment of the enrichment material absorption with phosphoric acid root label of phosphate radical is finally used, to obtain protein N end.It is an advantage of the invention that efficient guanidinated, phosphate-labeled and affinitive material enrichment so that the enrichment of albumen n end has excellent bioaccumulation efficiency and selectivity. |
| priorityDate | 2014-12-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 241.