| abstract |
The invention relates to a method of combining PMP (1-Phenyl-3-Methyl-5-Pyrazolone) derivatization and IM-MS/MS (Ion Mobility Tandem Mass Spectrometry), which solves the problem that an actually mixed sugar sample cannot be analyzed easily, quickly and accurately in the prior art for identifying oligosaccharide isomers, and meanwhile, solves the problem that the compositions of monosaccharide residues of the sugar cannot be determined by utilizing a mass spectroscopy means. According to the method for quickly identifying the sugar, the effects on identifying sugar isomers by taking PMP and PHN (Phenylhydrazine) as derivatization reagents and combining the IM-MS/MS method are compared, the CCS (Collision Cross Section) value of a derivatization product is calculated theoretically for further proving that mixed extracts of the sugar can be qualitatively identified successfully by combining the PMP derivatization and the IM-MS/MS method, and meanwhile, the connection, structures and compositions of the sugar can be identified. |