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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689
classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-689
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11
filingDate 2016-03-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2019-03-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2019-03-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-105695584-B
titleOfInvention The gyrB primer of test experience animal staphylococcus aureus combines
abstract The gyrB primer of test experience animal staphylococcus aureus combines, and belongs to experimental animal pathogenic microorganism detection technique field.The primer combination includes outer primer F3, B3, inner primer FIP, BIP, reaction system;Reaction system includes FIP, BIP, F3, B3,2 × reaction buffer, Bst archaeal dna polymerase, hydroxynaphthol blue HNB, template DNA and deionized water.The target gene of detection is the conservative specific gyrB sequence fragment of Staphylococcus aureus kind inner height.High specificity: the no positive amplification of negative control detected.High sensitivity, the minimum recall rate of genomic DNA are 1.4 × 10 ‐4 ng/μL.Result is convenient rapidly, out for detection: may occur in which positive findings in about 60min.It is easy to operate, it is low to instrument requirements, and can detect in practice and apply in base test experience animal center staphylococcus aureus by the direct observed result of dyestuff.
priorityDate 2016-03-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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