http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105695494-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2840-203 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-67 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-65 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-67 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65 |
| filingDate | 2016-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-02-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-02-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105695494-B |
| titleOfInvention | Three-cistron expression vector, preparation method and application |
| abstract | The invention discloses a three-cistron expression vector, a preparation method and application, belonging to the technical field of genetic engineering. The tricistronic expression vector comprises a nuclear matrix binding region sequence and a tricistronic sequence formed by connecting two internal ribosome entry site sequences, and the structure of the tricistronic sequence is as follows: promoter-light chain Signal peptide SP L IRESWt-heavy chain Signal peptide SP H IRESatt-selection marker-Poly A. The vector can simultaneously express the light chain, the heavy chain and the screening marker gene of an antibody, overcomes the problem of unbalanced expression of the heavy chain, the light chain and the screening marker gene of the traditional vector, improves the quality of the antibody and the screening rate of positive cell cloning, simultaneously overcomes the silencing of transgenes by a nuclear matrix binding region sequence contained on the vector, realizes the high-efficiency and long-term expression of the transgenes in host cells, improves the expression level of antibody protein on one hand, and improves the screening effectiveness of subsequent monoclonal antibody cell strains on the other hand. |
| priorityDate | 2016-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 283.