http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105647971-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-14043 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-866 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 |
| filingDate | 2016-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-05-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-05-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105647971-B |
| titleOfInvention | African swine fever P30 protein recombinant baculovirus expression vector and preparation method thereof |
| abstract | The invention provides an African swine fever P30 protein recombinant baculovirus expression vector and a preparation method thereof, wherein a P30 gene is amplified from a plasmid PCR-4TOPO-P30 containing an ASFV 30 full-length gene, the amplified P30 gene is connected to a baculovirus vector pFastBac1, a recombinant baculovirus vector pFastBac1-ASFV-P30 is constructed, the recombinant baculovirus vector is transformed into an escherichia coli competent cell DH10Bac, a recombinant shuttle plasmid rBacmid-ASFV-P30 is obtained, the recombinant baculovirus is transfected to an Sf9 insect cell after being correctly identified, the recombinant baculovirus is obtained, passage amplification is carried out on the recombinant baculovirus, and the High-titer baculovirus containing the ASFV P30 gene is connected to a High Five insect cell to carry out eukaryotic expression of an ASFV P30 protein. The method is selected to construct the African swine fever P30 protein recombinant baculovirus expression vector, and the recombinant baculovirus expression system is used for expressing the African swine fever P30 protein in insect cells, so that a foundation is laid for the establishment of the serological ELISA detection method for the African swine fever. |
| priorityDate | 2016-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 28.