http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105624304-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6841 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6841 |
| filingDate | 2016-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-01-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-01-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105624304-B |
| titleOfInvention | A kind of method for in situ hybridization of juvenile flounder gonadal mRNA |
| abstract | The invention relates to an in-situ hybridization technology of juvenile gonad mRNA, in particular to a method for in situ hybridization of gonad mRNA of juvenile flounder. The sample after washing was taken and fixed with a 4% PFA solution; after fixing, it was stored in 100% methanol solution at -20°C, and the above-mentioned fixed and preserved sample was processed as an in situ hybridization sample; the above in situ hybridization sample was rehydrated, Washing, proteinase K digestion, immobilization, washing, pre-hybridization, mixing with a hybridization solution containing a flounder RNA probe after pre-hybridization, and hybridizing overnight at 50-70° C.; washing after hybridization treatment, incubation with antibodies, and washing again, Avoid light for color development, stop color development, fix again, wash again, and settle with 20-30% sucrose; the sedimented samples are embedded in OCT and then frozen sectioned, so as to realize the labeling of in situ hybridization of flounder juvenile gonad mRNA. The implementation of the present invention can clearly describe the location (mRNA level) of the flounder related genes in the gonads, and has achieved a breakthrough. |
| priorityDate | 2016-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 51.