http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105624192-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2750-14143 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-195 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-70596 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-864 |
| filingDate | 2016-01-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105624192-B |
| titleOfInvention | Preparation of breast cancer cell lines that stably secrete near-infrared fluorescently labeled exosomes |
| abstract | The invention discloses a preparation method of a breast cancer cell line that stably secretes near-infrared fluorescence-labeled exosomes. By constructing a plasmid vector that fuses and expresses near-infrared fluorescence iRFP682 protein and CD63 protein, and then uses the plasmid to package a recombinant adenocarcinoma cell line. Human breast cancer MDA-MB-231 cells were co-infected with related virus (rAAV) and helper virus in a certain proportion, and the human breast cancer MDA-MB-231 cells that could stably express exosomes labeled with the near-infrared fluorescent protein iRFP682 were obtained by screening, and then The exosomes labeled with the near-infrared fluorescent protein iRFP682 were isolated and purified by step centrifugation for identification. The invention finally obtains a novel biomarker that can be used for in vivo and in vitro research of breast cancer tumor microenvironment, which provides a powerful tool for further research on the mechanism of intercellular material transfer, and also provides new support and opportunity for cancer diagnosis and treatment. |
| priorityDate | 2016-01-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 72.