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filingDate 2016-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2019-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2019-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-105624176-B
titleOfInvention It is overexpressed engineering bacteria and the building of UDPglucose pyrophosphorylase gene
abstract It is overexpressed engineering bacteria and the building of UDPglucose pyrophosphorylase gene, belongs to genetic engineering and technical field of microbial fermentation.The key enzyme UDPglucose pyrophosphorylase gene for having cloned polysaccharide flocculant route of synthesis, utilizes E. coli-Bacillus shuttle plasmid construction recombinant expression carrier.Recombinant plasmid is imported into bacillus licheniformis by the method for electrotransformation, construct recombination bacillus licheniformis HN301-2, for the recombination bacillus licheniformis of building than the polysaccharide flocculant output increased 15.6% of starting strain, fermentation liquid flocculation activity improves 70%.It is expected the industrialized production for polysaccharide flocculant, improves yield, reduce cost.
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