http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105624121-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-35 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-10 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-91 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-577 |
| filingDate | 2016-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105624121-B |
| titleOfInvention | Secrete anti-tomato black ring virus monoclonal antibody hybridoma cell strain and its monoclonal antibody application |
| abstract | The invention discloses a kind of applications for secreting anti-tomato black ring virus monoclonal antibody hybridoma cell strain and its monoclonal antibody.It is that BALB/c mouse is immunized in antigen with tomato black ring virus (TBRV) particle of purifying, through cell fusion, screening, clone, passage can be stablized and secrete anti-TBRV monoclonal antibody hybridoma cell strain 12H3 by obtaining 1 plant, and deposit number is CGMCC No.12005.The odd contradictive hydroperitoneum indirect ELISA titer of hybridoma cell strain secretion is up to 10 ‑7 , Antibody types and subclass are IgG1, kappa light chain.The monoclonal antibody and 50kDa TBRV coat protein have specific immune response.ACP-ELISA the and dot-ELISA method of TBRV in detection tomato is established using 12H3 monoclonal antibody, the sensitivity that both methods detects sick leaf respectively reaches 1:163,840 and 1:5,120 times of dilutions (w/v, g/mL).The acquisition of hybridoma cell strain 12H3 and its monoclonal antibody and the detection for being established as the virus and quarantine of Serology test and science bridle provide substance and technical support. |
| priorityDate | 2016-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 190.