http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105602984-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_af121e2320f1b2cc3010325ae9e3ada6 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8205 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 |
| filingDate | 2016-04-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_57b3738e26e6d9611ccf8edb811a9514 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c844e3fc93cd594a6522889cab14a315 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2261b7e234d479c6fa645ec4c1d3750f |
| publicationDate | 2016-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105602984-A |
| titleOfInvention | Rapid and efficient genetic transformation method for agrobacterium tumefaciens-mediated wheat stem tips |
| abstract | The invention discloses a rapid and efficient genetic transformation method for agrobacterium tumefaciens-mediated wheat stem tips. The method mainly comprises the steps of carrying out water seed soaking, accelerating germination and cold treatment on mature wheat seeds, transversely cutting coleoptiles of the wheat seeds until exposing stem tips, taking the cut wheat seeds as a transformation acceptor material, carrying out genetic transformation by virtue of vacuum treatment mediation on agrobacterium tumefaciens, placing the transformed material in a suitable growth environment, and carrying out transgenic identification after the plants grow, so as to obtain transgenic wheat plants. By virtue of the rapid and efficient genetic transformation method, the culture of callus tissues of the wheat is omitted, so that the influences caused by various factors to the genetic transformation of the wheat are reduced, and the problems of vitrification, browning, cell mutation and the like in the tissue culture process are avoided; the genetic transformation conditions of the wheat are optimized, so that the economic cost of the genetic transformation of the wheat is lowered, the restriction of the genotype of the wheat to the transformation efficiency is effectively overcome, and a large amount of transgenic wheat plants can be rapidly obtained; and the rapid and efficient genetic transformation method has significant values to the culture of high-quality new-variety wheat and the research of the genetic transformation of the wheat. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115088619-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105925552-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110656129-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111713288-A |
| priorityDate | 2016-04-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 34.