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http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P17-02
classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70
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filingDate 2016-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2018-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2018-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-105602916-B
titleOfInvention A kind of DBAT mutant enzymes R363H and its application
abstract The present invention discloses a kind of DBAT mutant enzymes R363H and its application.The mutant enzyme is that amino acid sequence is SEQ ID NO:363rd amino acids of 2 DBAT enzymes change into His by Arg;Its amino acid sequence is SEQ ID NO:14.The present invention successfully constructs mutant enzyme R363H.The mutant enzyme improves 12 times on the catalytic efficiency using acetyl coenzyme A than wild-type enzyme, substantially increases the yield of the utilization rate and product Bakating III of acetyl coenzyme A.Meanwhile to reduce production cost, the present invention has found 7 kinds of novel acry radical donors, and mutant enzyme can efficiently utilize these acry radical donors, wherein improving 100 times to the utilization rate of vinyl acetate, can be used to prepare Bakating III as expensive acetyl coenzyme A is substituted.So far, in the research of biosynthesis Bakating III, and find no using non-plant coenzyme class as acry radical donor and embody the research of high usage.
priorityDate 2016-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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Total number of triples: 48.