http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105602913-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P17-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0006 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y101-01184 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P17-12 |
| filingDate | 2016-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-02-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-02-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105602913-B |
| titleOfInvention | Recombinant carbonyl reductase mutant ReCR-Mut, encoding gene, engineering bacteria and application |
| abstract | The invention discloses a recombinant carbonyl reductase mutant ReCR-Mut, an encoding gene, an engineering bacterium and an application thereof. The mutant ReCR-Mut is the amino acid sequence shown in SEQ ID NO. Mutated to phenylalanine; mutant ReCR-Mut has higher enzymatic activity and catalytic efficiency than wild-type ReCR; specific enzyme activity of mutant ReCR-Mut reducing N-Boc-3-piperidone It is 1.42 times that of wild-type ReCR, and the k cat /K m value of mutant ReCR-Mut to N-Boc-3-piperidone is 1.37 times that of wild-type; Alcohol is used as co-substrate and organic phase, and NAD + is used as coenzyme to synthesize optically pure (S)-N-Boc-3-piperidinol efficiently and asymmetrically; in the preferred two-phase system, 300g/LN-Boc-3-piperidol The asymmetric reduction reaction of the pyridone was catalyzed by the whole cell method for 12 hours, the product ee value of the product (S)-N-Boc-3-piperidinol was >99%, and the yield was 93.9%; and the wild-type carbonyl reductase ReCR Compared with the biocatalyst, the product yield increased by 11.9%. |
| priorityDate | 2016-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 73.