http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105601749-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d94573ab2c57e8b193f3a22accddf9fe |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-24 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-01004 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-18 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 |
| filingDate | 2016-01-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_238c87815abcc546e153e5a4933cc5c8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b8184fb128430fa50e4360cafa754ddd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d746a9db358eade2a5f3936d30422ffa |
| publicationDate | 2016-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105601749-A |
| titleOfInvention | A recombinant clonorchis sinensis secreted phospholipase A2 protein and its in vitro soluble expression and purification preparation method |
| abstract | The invention belongs to the field of biotechnology, and specifically relates to a recombinant clonorchis sinensis secreted phospholipase A2 protein and its in vitro soluble expression and purification preparation method. <i>Cs</i>sPLA2 obtained by existing expression technology? The protein is an inclusion body, which has no enzymatic activity, and the enzymatic activity is very weak even after renaturation of the inclusion body. The recombinant <i>Cs</i>sPLA2 of the present invention? protein, containing <i>Cs</i>sPLA2? ? protein and MBP-tagged protein. The present invention, through extensive and in-depth research, through the optimization of special expression,? First implementation of <i>Cs</i>sPLA2? Soluble expression of protein, and preparation of recombinant <i>Cs</i>sPLA2 with biological activity? protein, which effectively overcomes the problems existing in the existing expression technology. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110272499-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106834327-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110272499-A |
| priorityDate | 2016-01-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 67.