http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105572258-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-027 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-02 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 |
filingDate | 2016-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2017-11-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2017-11-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-105572258-B |
titleOfInvention | Orobanche pycnostachya Hance fingerprint |
abstract | The present invention relates to the method for building up of orobanche pycnostachya Hance finger-print, including:1) preparation of need testing solution:Orobanche pycnostachya Hance medicinal powder, plus 70% methanol are taken, weighed weight, immersion is ultrasonically treated, and the weight of less loss is supplied with solvent, filters, takes subsequent filtrate to be filtered with 0.45 μm of miillpore filter;2) need testing solution is injected in high performance liquid chromatograph, obtains finger-print, wherein, chromatographic column:Octadecylsilane chemically bonded silica chromatographic column;Mobile phase:The phosphate aqueous solution (B) of acetonitrile (A) 1 ‰, gradient elution 89%B (0min)~85%B (13min)~81%B (22min)~78%B (33min)~76%B (50) min;Flow velocity:1ml/min;230~400nm of Detection wavelength;20~30 DEG C of column temperature.The present invention also provides a kind of method that utilization finger-print evaluates orobanche pycnostachya Hance quality of medicinal material.High, the favorable reproducibility according to the orobanche pycnostachya Hance fingerprint analysis method sensitivity that the present invention is set up;The standard finger-print obtained can more comprehensively reflect chemical composition species and quantity contained in orobanche pycnostachya Hance medicinal material. |
priorityDate | 2016-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 50.