http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105572234-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 |
| filingDate | 2014-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-11-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-11-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105572234-B |
| titleOfInvention | A kind of method for rapidly and efficiently determining Chinese small iris cysteine and glutathione content |
| abstract | A kind of method for rapidly and efficiently determining Chinese small iris cysteine and glutathione content, belongs to analysis determining technology field.This research and utilization ultra performance liquid chromatography（UPLC）, to cysteine in Chinese small iris extract solution（Cys）And glutathione（GSH）Carry out qualitative and quantitative analysis：Vydac C18 chromatographic columns are chosen, mobile phase is 0.1% TFA（A）With 100% ACN（B）；30 DEG C of column temperature, flow velocity 0.5mlmin ‑1 , sample size is 1 μ l；Gradient elution program：12%~25% ACN（3min）, 25%~100% ACN（0.5min）；Wash post：100% ACN（1.5min）；Column equilibration：100%~0% ACN（0.5min）With the min of flowing time after post 1.5.Fluorescence detector condition is：λ ex （Excitation wavelength）380nm、λ em （Launch wavelength）470 nm.Quantified by external standard method is carried out with peak area, Cys and GSH can be tested and analyzed simultaneously in 7min.This method and traditional high performance liquid chromatography（HPLC）Compare, analysis process greatly shortens, good separation, greatly improves detection efficiency and saves mobile phase cost. |
| priorityDate | 2014-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 23.