http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105567850-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6886 |
| filingDate | 2016-02-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105567850-B |
| titleOfInvention | For quantitative detection RPRM gene DNA methylating reagent box and method |
| abstract | The present invention provides be used for quantitative detection RPRM gene DNA methylating reagent box and method, the kit includes: bisulfite conversion reaction solution, glass fibre membrane centrifugal column, combination buffer, wash buffer, desulfurization buffer, elution buffer, 2 × SYBR GREEN I premix reaction solution, primer RPRM-F and RPRM-R, methylation positive control plasmid S M And non-methylation positive control plasmid S U .It completes to convert the bisulfite of DNA in 2 hours, after purified, PCR amplification is carried out by the RPRM gene DNA region after special primer pair conversion, then the melting curve analysis (MS-MCA) for carrying out methyl-sensitive, to detect whether the degree of methylation and the methylation of RPRM gene.This method is detection sample with blood plasma, and operation sequence is greatly simplified, high sensitivity, specificity is good, testing result is stable, there is important application prospect. |
| priorityDate | 2016-02-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 48.