http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105567739-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-16043 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2750-14143 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2740-15043 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-10043 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K45-06 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-864 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-867 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-861 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P29-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K45-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P19-02 |
| filingDate | 2016-02-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105567739-B |
| titleOfInvention | Vector particles and its construction method and application |
| abstract | The invention belongs to field of biomedicine technology, and in particular to a kind of vector particles and its construction method and the application in treatment human chronic's pain medication.The vector particles include pspax2, pmd2.G and pwpi plasmid, and the pwpi plasmid includes promoter, creotoxin enzyme activity area and internal ribosome entry site sequence.The construction method of the vector particles includes the culture of (1) cell line lenti-x293T；(2) DMEM culture medium is changed into；(3) by pspax2, pmd2.G and pwpi plasmid transfection to lenti-x293T cell；(4) transfection liquid is washed off, the culture solution that the calf serum containing 10wt% is added is cultivated；(5) culture solution is recycled, filtering, centrifugation can be obtained the vector particles of high titre.Vector particles of the present invention are more targeted to treatment chronic ache, can prevent the vicious circle between nervous system and peripheral cells. |
| priorityDate | 2016-02-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 470.