http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105567733-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-30 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-6429 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-21005 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-74 |
| filingDate | 2016-01-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105567733-B |
| titleOfInvention | Method for expressing thrombin-Fc fusion protein in CHO cells |
| abstract | The present invention provides a method for expressing thrombin-Fc fusion protein in CHO cells, comprising the following steps: (1) constructing an expression plasmid: constructing and expressing thrombin a-subunit and b-subunit and human IgG1 monoclonal antibody respectively The recombinant expression vector plasmid of the Fc segment of the invariant region; (2) cell transfection: CHO cells are transfected with the above recombinant expression vector plasmid; (3) the above CHO cells are acclimated and cultured, and the supernatant is collected. The method of the invention efficiently and stably expresses the double-subunit fusion protein with enzymatic activity, that is, the CHO cell expresses the thrombin-Fc fusion protein. |
| priorityDate | 2016-01-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 31.