http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105548167-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-33 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-78 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-33 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78 |
filingDate | 2015-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2019-05-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2019-05-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-105548167-B |
titleOfInvention | Manganese dioxide thin slice simulates the detection method of enzyme sensor and preparation method and T4PNK |
abstract | The invention discloses a kind of manganese dioxide thin slice simulation enzyme sensor and the detection method of preparation method and T4PNK, the preparation methods, comprising: 1) by MnO 2 Nanometer sheet solution and TMB (3,3', 5,5'- tetramethyl benzidine) solution are mixed so that MnO is made 2 - TMB mixed solution;2) hair clip DNA is dissolved in Tris-HCl buffer solution, ATP (atriphos) and λ EXO (λ excision enzyme) is added then to be mixed to prepare DNA solution;3) by MnO 2 - TMB mixed solution is mixed with DNA solution manganese dioxide thin slice is made and simulates enzyme sensor.The preparation method is easy to operate, while sensor obtained has the characteristics that high sensitivity, detection limit are low and easy to operate for the detection of T4PNK. |
priorityDate | 2015-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 55.