http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105548050-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-31 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-31 |
filingDate | 2016-01-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2019-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2019-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-105548050-B |
titleOfInvention | A method of utilizing cromoci self assembly peroxidase determination critical micelle concentration of surfactant |
abstract | The invention discloses a kind of methods using cell sanguinin C self assembly peroxidase determination critical micelle concentration of surfactant, it includes the following steps: that the surfactant for 1) taking different quality is dissolved in phosphate buffer respectively, obtains a series of surfactant solutions with a certain concentration gradient;2) cromoci, guaiacol and hydrogen peroxide are added into each solution obtained by step 1), in 25-50 DEG C of beginning enzymatic reaction, records guaiacol and aoxidizes initial velocity;3) using surfactant concentration as abscissa, it maps using guaiacol oxidation initial velocity as ordinate, add rolling average Trendline power Trendline and rolling average Trendline, keep rolling average Trendline and power Trendline tangent by changing the rolling average period in rolling average Trendline, the corresponding surfactant concentration in point of contact is critical micelle concentration.The advantages that present invention has work pH, wide temperature range, easy to operate, high sensitivity. |
priorityDate | 2016-01-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 38.