http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105543399-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858 |
| filingDate | 2016-02-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105543399-B |
| titleOfInvention | A method of utilizing fluorescence detection microcystis DNA damage |
| abstract | The present invention discloses a kind of method using fluorescence detection microcystis DNA damage, step :(a) sample to be tested and control sample are divided into three groups respectively；(b) it removes cell keratinized sheath: frustule is collected by centrifugation in sample and is resuspended in SE buffer and washs；(c) cell: being resuspended to Lysis lysate by cell cracking respectively, and Proteinase K and dodecyl sodium sulfate is added, makes cell cracking；(d) cell DNA chain untwists: changing pH, T, P and B sample is made to untwist at different conditions；(e) it dyes: Hoechest 33258 being added into above T, P and B sample respectively and dyes；(f) fluorescence intensity of supernatant fluoremetry: is detected after centrifugation in fluorescence detector；(g) result calculates: the fragmentation levels of DNA chain are calculated according to the fluorescence of T, B and P sample in sample to be tested and control sample.This method is easy to grasp, and high sensitivity, and single broken site can be detected in DNA chain. |
| priorityDate | 2016-02-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 65.