| abstract |
The invention discloses application of a potato leaf roll virus recombination CP antibody in preparing a test paper strip. pET22b(+) is adopted as an initial carrier, so that the expression efficiency of a PLRV-CP gene is obviously improved; by the adoption of a 5mL protein purification column, the purification efficiency is improved, and the purification difficulty is lowered (the column is not prone to blockage), the expression product is 20 kDa (originally 34kDa), only a His label exists, no other fusion segment exists, and possible interference of the fusion segments on antibody preparation is reduced. A PLRVP-CP efficient expression and large-scale preparation system is built, and a solid foundation is laid for polyclonal antibody and monoclonal antibody preparation. In addition, the research of the test paper strip lays a foundation for simplification and high-efficiency implementation of specific detection procedures. |