http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105543177-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-35 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-10 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-91 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-577 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 |
| filingDate | 2016-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-01-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-01-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105543177-B |
| titleOfInvention | Secrete hybridoma cell strain and its monoclonal antibody application of anti-citrus yellow vein clearing virus monoclonal antibody |
| abstract | The invention discloses the applications of the hybridoma cell strain and its monoclonal antibody of a kind of anti-citrus yellow vein clearing virus (CYVCV) monoclonal antibody of secretion.Coat protein using the citrus yellow vein clearing virus of prokaryotic expression is that BALB/c mouse is immunized in antigen, through cell fusion, screening, clone, the hybridoma cell strain 18H5 for passing on and secreting anti-CYVCV monoclonal antibody can be stablized by obtaining 1 plant, and deposit number is CGMCC No.12003.The cell strain secretes monoclonal antibody ascites indirect ELISA titer up to 10 ‑7 , Antibody types and subclass are IgG1, and the coat protein subunit of kappa light chain, the monoclonal antibody and CYVCV 32kD has idiosyncrasy.TAS-ELISA, dot-ELISA and Tissue blot-ELISA detection method of CYVCV in detection mandarin tree is established using 18H5 monoclonal antibody, the sensitivity that wherein TAS-ELISA and dot-ELISA method detects sick leaf respectively reaches 1:2560 and 1:20480 times of dilution (w/v, g/mL).The preparation of anti-CYVCV monoclonal antibody and its diagnosis for being established as the citrus virosis and detection of detection method, epidemiological analysis and science bridle provide substance and technical support. |
| priorityDate | 2016-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 232.