| abstract |
The invention discloses a preparation method of Vgamma9Vdelta2T cell preparation for treating multiple myeloma. The method comprises the following steps that 1, peripheral blood mononuclear cells from a patient are isolated; 2, Vgamma9Vdelta2T cell stimulation and amplification culture are conducted; 3, detection of the cell number, the survival rate, sterility, endotoxin, acid resistance and immunophenotype of Vgamma9Vdelta2T cells cultured for 11 or 12 days is conducted; 4, the Vgamma9Vdelta2T cells are collected on the 13<th> day or the 14<th> day, an IL-12 adjuvant of 5-15 ng/ml is added, and the Vgamma9Vdelta2T cell preparation can be obtained. The Vgamma9Vdelta2T cell preparation can obviously improve the cytotoxicity and tumor-killing capacity and is used in drugs or biological products for treating multiple myeloma. |