http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105532583-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-033 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-033 |
| filingDate | 2015-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-04-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-04-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105532583-B |
| titleOfInvention | A kind of living body propagation method of entomopathogenic nematode |
| abstract | The present invention relates to a kind of living body propagation method of entomopathogenic nematode, it comprises the following steps：Culture vessel is taken, sponge block is put into the culture vessel, sterilized water is added dropwise to sponge block complete wetting to sponge block；The appropriate entomopathogenic nematode aqueous solution is dropped in 12~24h of the starvation high instar larvae body surface of beet armyworm, the high instar larvae of the beet armyworm is placed on sponge block after 1~3min；Culture vessel is positioned over it is incubated in 22~25 DEG C of incubators, in incubation keep sponge block be continuously in complete moisture state；After culture 10~15 days, pathogenic nematode is distributed in sponge block, required entomopathogenic nematode is collected with sterile water wash sponge block.Present invention breeding equipment is simple, it is low to reduce living body propagation cost, it is simple to operate, host insect, source is wide, convenient collection, using sponge block as breeding carrier, the expelling water of nematode is reused, pathogenic nematode effectively infecting to the high instar larvae of beet armyworm is ensure that, is further ensured that the yield of pathogenic nematode. |
| priorityDate | 2015-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
| Predicate | Subject |
|---|---|
| isDiscussedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID962 http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID419512635 |
Total number of triples: 12.