http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105527332-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-44778 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-447 |
| filingDate | 2016-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-05-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-05-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105527332-B |
| titleOfInvention | The extraction of lily total protein and the acquisition methods of dielectrophoresis differential protein collection of illustrative plates |
| abstract | The present invention discloses a kind of extraction of lily total protein and the acquisition methods of dielectrophoresis differential protein collection of illustrative plates.This method sets rotating speed as 20000rpm in the extraction of lily gross protein, and repeated precipitation centrifuges at least 3 times so that the lily gross protein color extracted is whiter, and quality is good；Add the quality inspection steps of protein, the mode for running sodium dodecyl sulfate polyacrylamide gel taken to detect the quality of extracted protein, avoid follow-up first to isoelectric focusing effect it is bad the problem of.Improve first during isoelectric focusing focalizer, by the time lengthening of step two salt-removal steps of S1 and S2 in focalizer to 2.5h, the salinity of protein is preferably eliminated, so as to preferably complete focalizer.The lily total protein dielectrophoresis differential protein collection of illustrative plates clear background that the method for the present invention obtains, protein spots are more, and protein spots are evenly distributed. |
| priorityDate | 2016-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 150.