http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105524978-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-37 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-37 |
| filingDate | 2016-02-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-01-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-01-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105524978-B |
| titleOfInvention | A method of detection extracellular protease Caseinolytic activity and heat resistance |
| abstract | The present invention provides a kind of detection extracellular protease Caseinolytic activity and the methods of heat resistance.This method comprises: microbial culture medium is centrifuged, takes supernatant by (1)；(2) a part of supernatant is heated；(3) will by mix with reaction buffer with the supernatant without Overheating Treatment, 35-45 DEG C reaction 2-3 hour, terminate and react, be centrifuged, supernatant is taken to be transferred to porous plate, addition strong base solution, detection 450nm light absorption value；(4) 450nm light absorption value is scaled extracellular protease Caseinolytic activity；The heat resistance of extracellular protease is the ratio through the extracellular protease Caseinolytic activity in Overheating Treatment and the supernatant without Overheating Treatment.The method of the present invention can carry out quantitative test to extracellular microbial exoproteinase Caseinolytic activity and heat resistance simultaneously, difference between more accurate relatively bacterial strain, keep operation more convenient, saves culture medium, can be realized batch operation and can directly detect supernatant of bacteria solution. |
| priorityDate | 2016-02-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 26.