http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105524903-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01021 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2445 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-84 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 |
| filingDate | 2016-01-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105524903-B |
| titleOfInvention | A kind of beta-glucosidase improvement mutant E168Q and its encoding gene and application |
| abstract | The present invention relates to genetic engineerings and field of genetic engineering, in particular it relates to a kind of beta-glucosidase improvement mutant E168Q and its encoding gene and application.The present invention is expressed after carrying out rite-directed mutagenesis to beta-glucosidase enzyme sequence using Protocols in Molecular Biology with being female parent from the high-temperature acidic beta-glucosidase BGL3A of the basket bacterium Talaromyces leycettanus JCM12802 of thermophilic fungal.Under the conditions of being transformed herein, the affinity to cellobiose of mutant improves 2.0 times than wild type (before mutation), and catalytic efficiency improves 1.5 times, and optimal reaction pH value and temperature-resistant.The catalytic efficiency of beta-glucosidase can be greatly improved using this strategy, provide application foundation in field of industrial production such as food, bio-ethanols for it.This strategy, which improves the catalytic efficiency of beta-glucosidase and other enzymes, has important directive significance. |
| priorityDate | 2016-01-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 126.