http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105519434-B

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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-008
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filingDate 2015-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2017-11-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2017-11-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-105519434-B
titleOfInvention A kind of tissue-cultured seedling micro-grafting method of Japanese maple orange dream
abstract The present invention discloses a kind of tissue-cultured seedling micro-grafting method of Japanese maple orange dream, including (1) sterile stock is cultivated:Fructus Aceris elegantulis band bud branch section is cut, tissue cultures is carried out, obtains Fructus Aceris elegantulis aseptic seedling, the beveling of Fructus Aceris elegantulis aseptic seedling is blocked, obtains sterile stock;(2) sterile scion cultivated:Japanese maple orange dream band bud branch section is cut, tissue cultures is carried out, obtains Japanese maple orange dream aseptic seedling, the beveling of Japanese maple orange dream aseptic seedling is blocked, obtains sterile scion;(3) tissue-cultured seedling micrografting:The oblique section of sterile stock is alignd with the oblique section of sterile scion, and it is fixed, micrografting seedling is obtained, the grafting is inoculated on culture medium and cultivated;(4) acclimatization and transplantses:Micrografting seedling obtained by step (3) is transferred to greenhouse hardening at initial stage, then is transferred to hardening in seedbed, spraying treatment and sterilization processing, hardening can transplant after terminating;Methods described can improve grafting efficiency, improve reproductive number, survival rate is high, can carry out cultivating small dimension Japan maple orange dream seedling on a large scale.
priorityDate 2015-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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