http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105506125-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-06 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B40-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06 |
filingDate | 2016-01-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2019-01-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2019-01-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-105506125-B |
titleOfInvention | A kind of sequencing approach and a kind of two generation sequencing libraries of DNA |
abstract | The invention discloses the sequencing approach of DNA a kind of and a kind of two generation sequencing libraries.Sequencing approach of the invention carries out extension by DNA fragmentation to be measured and random primer Read2-NB mixing, by the b chain link at 3 ' ends and the distinguished sequence containing microarray dataset in double-stranded adapters Read1-Adapter of gained extension products, specific primer T1 and T2 is taken to expand connection product, to complete the building of two generation sequencing libraries, then it is sequenced.The present invention can carry out library construction and sequencing to double-stranded DNA and/or single stranded DNA, and required starting amount of DNA reduces, strong for the adaptive type of the limited sample of template DNA, and can directly distinguish target single stranded DNA primary template sequence from sequencing result. |
priorityDate | 2016-01-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 22.