http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105505860-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-05 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-25 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-727 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-998 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-155 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-999 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0602 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-074 |
| filingDate | 2016-01-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105505860-B |
| titleOfInvention | Separation culture method of esophageal epithelial stem cells |
| abstract | The invention discloses a separation culture method of esophageal epithelial stem cells, which comprises the following steps: firstly, taking esophageal mucosa tissue and attached epithelium, removing muscle tissue, taking a basal layer of the mucosal epithelium tissue, shearing the basal layer into fragments, digesting and centrifuging, and paving the obtained cells in a culture bottle, a culture plate or a culture dish coated with a matrix; then adding DMEM/Ham's F-12 culture solution added with HEPES, sodium bicarbonate, L-glutamic acid, FBS, EGF, insulin, hydrocortisone, Vibrio cholerae toxin, transferrin, BPE, retinoid and cell signaling pathway inhibitor; and finally, placing a culture bottle, a culture plate or a culture dish containing the cell suspension into a 37 ℃ incubator for culture, and forming the monolayer epithelial stem cells after 10-14 days. The method is simple and convenient to operate, has good safety, can separate and obtain the esophageal epithelial stem cells, has high activity of the stem cells, and has good application prospect in the fields of tissue engineering and regenerative medicine. |
| priorityDate | 2016-01-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 184.