abstract |
The invention discloses a method for raising seedlings of masson pine tissue culture seedlings with short branches and short spikes, including container selection, substrate preparation, cuttings collection, cuttings treatment, cuttings and nursery management procedures, selecting a suitable seedling container, and disinfecting according to a certain ratio Method After the substrate is prepared, the juvenile shoots are collected as cuttings and washed and disinfected. After being treated with a root promoting agent, the cuttings are directly cut. Reached more than 98%, and the survival rate reached more than 97%. The present invention uses the tissue-cultured seedlings of Pinus massoniana as the mother plant for cutting, on the one hand, it overcomes the problems of long tissue culture subculture cycle and high cost of seedling cultivation, and at the same time, the reproduction multiple is high, and the number of qualified juvenile spikes available for cutting It is very easy to cut and take root, the effect is remarkable, and it has good economic, social and ecological benefits. |