| abstract |
The invention discloses a method for obtaining pancreatic beta cells through induced differentiation of human umbilical cord mesenchymal stem cells through a gene transfer method. Pancreatic cell specific genes containing PDX1, MAFA, NGN3, FOXA2, NEUROD1 and FGF21 are used, adenovirus expression vectors of transmembrane singles are connected, various recombinant protein and small molecules are combined, and the human umbilical cord mesenchymal stem cells are induced to be directionally differentiated into the pancreatic beta cells. The inducing method is easy and convenient to implement, simple in operation and good in differentiation effect. |